type reference a baumannii strain atcc 19606  (ATCC)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antibacterial activity of enmetazobactam against Acinetobacter spp.: a molecular dissection of mechanism of action and resistance determinants

doi: 10.1128/aac.01206-25

Figure Lengend Snippet: Representative assay of a PBP-binding IC 50 SDS-polyacrylamide gel with increasing amounts of sulbactam and enmetazobactam in competition with Bocillin FL. The identified PBPs of the A. baumannii strain ATCC 19606 are indicated in the figure.

Article Snippet: Thus, the capacity of enmetazobactam and sulbactam (comparator) to bind to PBPs, reported as the 50% inhibitory concentration (IC 50 ), was assessed in the wild-type reference A. baumannii strain ATCC 19606.

Techniques: Binding Assay

Journal: mSphere

Article Title: Genomic and phenotypic analysis of ST25 A. baumannii identifies virulence-associated clades and capsular/outer core locus types

doi: 10.1128/msphere.00717-25

Figure Lengend Snippet: Serum resistance of ST25 A. baumannii , ST2 ACICU, ST1 AYE, and ST52 ATCC 19606. The viable cells (CFU/mL) were determined for each isolate following a 5- to 60-min incubation in 20% activated serum and normalized using values obtained from incubation with heat-inactivated. The data were obtained from three independent experiments in which each isolate was tested in triplicate. *** P -values <0.001.

Article Snippet: To investigate the role of EPs on virulence features of ST25 A. baumannii , a set of isogenic mutants derived from the A. baumannii ATCC 19606 wild-type strain was used.

Techniques: Incubation

Journal: Frontiers in Pharmacology

Article Title: Bakuchiol kills Staphylococcus aureus persisters and potentiates colistin activity against Acinetobacter baumannii persisters

doi: 10.3389/fphar.2025.1592183

Figure Lengend Snippet: Bakuchiol induces membrane permeabilization but does not inhibit growth or cause ATP leakage in Acinetobacter baumannii due to the presence of LOS. (A) Growth of wild-type Acinetobacter baumannii ATCC 19606 and its lipooligosaccharide-deficient mutant (LpxC mutant) treated with varying concentrations of bakuchiol or colistin for 18 h. Bacterial growth was assessed by measuring the optical density at 600 nm (OD 600 ). Data are presented as means ± SD (n = 3). (B) Membrane permeabilization assessed by SYTOX Green uptake (Ex = 485 nm, Em = 525 nm) in wild-type Acinetobacter baumannii ATCC 19606 and LpxC mutant treated with the indicated concentrations of bakuchiol or colistin. Data are presented as means (n = 3 biologically independent samples); error bars are omitted for clarity. RFU indicates relative fluorescence units. (C) Intracellular ATP leakage from wild-type Acinetobacter baumannii and the LpxC mutant treated with varying concentrations of bakuchiol (BAK) or colistin (COL) for 1 h, measured using an ATP luminescence assay kit. Individual data points are shown, with error bars representing means ± SD (n = 3). Statistical significance was analyzed using two-way analysis of variance with Tukey’s post hoc test (* p < 0.05, ** p < 0.01).

Article Snippet: We compared the antimicrobial activity of bakuchiol between wild-type A. baumannii ATCC 19606, which has an intact outer membrane, and the LpxC mutant.

Techniques: Membrane, Mutagenesis, Fluorescence, Luminescence Assay

Journal: Frontiers in Pharmacology

Article Title: Bakuchiol kills Staphylococcus aureus persisters and potentiates colistin activity against Acinetobacter baumannii persisters

doi: 10.3389/fphar.2025.1592183

Figure Lengend Snippet: Bakuchiol and colistin exhibit synergistic bactericidal activity against Acinetobacter baumannii persisters. (A) Acinetobacter baumannii persisters were treated with 100× MIC ciprofloxacin (Cipro), meropenem (Mem), or gentamicin (Gm) for 4 h, and the titer of viable cells was determined. No decrease in viability confirms the antibiotic tolerance of Acinetobacter baumannii persisters. (B) Acinetobacter baumannii persister cells were challenged with the indicated concentrations of bakuchiol, colistin, or their combination. Data points at the x-axis detection limit represent a CFU count of 2 × 10 2 CFU/mL. Individual data points (n = 3 biologically independent samples) are shown, with error bars representing means ± SD.

Article Snippet: We compared the antimicrobial activity of bakuchiol between wild-type A. baumannii ATCC 19606, which has an intact outer membrane, and the LpxC mutant.

Techniques: Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Bakuchiol kills Staphylococcus aureus persisters and potentiates colistin activity against Acinetobacter baumannii persisters

doi: 10.3389/fphar.2025.1592183

Figure Lengend Snippet: Bakuchiol restores the membrane-disrupting activity of colistin against Acinetobacter baumannii persisters. (A) Uptake of SYTOX Green (Ex = 485 nm, Em = 525 nm) by Acinetobacter baumannii ATCC 19606 persister cells treated with colistin (COL), bakuchiol (BAK), or their combination at the indicated concentrations. Results are representative of three independent experiments. RFU denotes relative fluorescence units. (B) Intracellular ATP leakage from Acinetobacter baumannii persisters treated with COL, BAK, or their combination for 1 h at the indicated concentrations, measured using an ATP luminescence assay kit. Individual data points are shown, and error bars represent means ± SD (n = 3). RLU denotes relative luminescence units. Statistical significance was analyzed using one-way analysis of variance with Tukey’s post hoc test (* p < 0.05, *** p < 0.001).

Article Snippet: We compared the antimicrobial activity of bakuchiol between wild-type A. baumannii ATCC 19606, which has an intact outer membrane, and the LpxC mutant.

Techniques: Membrane, Activity Assay, Fluorescence, Luminescence Assay